This research investigates into the development of AI based techniques for classification of multi-category diseases using gene expression profile and next generation sequencing (NGS) data. Many diseases including Cancers involve malfunctioning of genes that control cell growth and division. The variation in expression values in certain genes contains key information about those diseases. The expression levels of thousands of genes can be measured simultaneously using a technique called Gene Expression profiling and this microarray data then can be used to predict and classify diseases, which will subsequently make treatment easy. Similarly, NGS data contains epigenetic influence on cancer development and classification of cancer can be carried out based on this information.