Prof Martin Knight
BEng, MSc, PhD, FHEA
Research Group News
Prof Knight is excited to be speaking on an expert panel at the upcoming 10th PREDiCT: Tumour Models London Summit which is uniting preclinical and translational leaders to narrow the preclinical gap and progress efficacious candidates to the clinic. He will be presenting on 'Organ-on-a-chip Models of Bone Metastasis' and … [more]
Prof Martin Knight was part of an expert panel at the 'lead the debate' event organised by the Royal Society for the Prevention of Cruelty to Animals (RSPCA). The event was the second in the series, which aims to convene small groups of thought leaders on prominent topics affecting … [more]
We have recently published two new papers in the journal iScience, which describe the development of novel multi-cellular in vitro models of ovarian cancer and the use of these models to better understand this disease. The work stems from a major European Research Council grant, CANBUILD, which was led … [more]
New organ-on-a-chip study demonstrates how physical exercise regulates bone metastasis in breast and prostate cancer17 June 2021
Bioengineers at Queen Mary publish a new study in the journal Cancers which demonstrates the significant impact of physiological mechanical loading on bone metastasis in breast and prostate cancer. The paper describes a new organ-chip model of bone metastasis which incorporates cancer cells and bone cells in … [more]
Congratulations to Eisha Hashmi who was selected for the IMechE Vicon Award for the best Bioengineering undergraduate project. Eisha presented her 3rd year individual research project at the Healthcare Technologies Student and Early Career Awards event held by Institute of Mechanical Engineers (IMechE) and was awared the … [more]
As part of the Science & Engineering Faculty’s mission to transfer horizons for all students, the School Management Team are delighted to report that SEMS have won four S&E Summer Research Internships for BAME Undergraduate Students. Following a rigorous selection process examining the quality of the … [more]
Congratulations to Dr Clare Thompson who has published a new paper on the role of polycystin in chondrocyte mechanotransduction. The study published in the International Journal of Molecular Science in a special issue on Mechanobiology of Organelles. In the paper we subjected isolated chondrocytes to mechanical stimulation and … [more]
Intercalated student gives symposium on her research project at Royal National Orthopaedic Hospital.22 March 2021
Congratulations to Elisha Koonjul, who gave an excellent symposium presentation on her iBSc research project to clinicians at the Royal National Orthopaedic Hospital (RNOH). Elisha's project investigates the effect of catheter clamping on the capacity and biomechanics of urinary bladder in patients with a spinal cord injury. This technique … [more]
Great to return to the campus last week and see some of the research team. So nice to see people in person after a year on Microsoft Teams! I toured the new PhD shared study space, which looks fantastic and a great improvement. Also nice to see the new ground … [more]
Steve Mould, the famous science communicator and YouTuber, has just produced a new video on the physics behind the shape of the urine stream. Steve explains that there is very little published literature on this phenomenum but he describes one key paper ... ... In 2012 Prof Knight and colleagues published a paper … [more]
Knight's group publish new paper on the importance of mechanical stimulation in organ-chip technology24 February 2021
Prof Knight and colleagues at Queen Mary University of London and University College Dublin have published a new paper discussing the importance of incorporating mechanical stimulation into organ-on-a-chip technology. Prof Knight will be discussing this work at the forthcoming Pan-London 3Rs Symposium. Organ-chip models consist of living cells grown in … [more]
Booking is now open for the Inaugural QMUL Festival of Education (https://www.qmul.ac.uk/queenmaryacademy/festival-of-education-2021/) will take place 8-12th March. Details on the Festival Agenda and how to book places for talks can be found on the SEMS Events pages (https://www.sems.qmul.ac.uk/… [more]
A new research grant using organ-on-a-chip technology to understand breast cancer bone metastasis has started in Prof Martin Knight's bioengineering and mechanobiology research group. The multidisciplinary award is jointly funded by Cancer Research UK (CR UK) and the Engineering and Physical Sciences Research Council (EPSRC). The project will develop … [more]
Prof Knight's research group have published a new study showing, for the first time, the anti-inflammatory effects of stimulating the mechanosensitive ion-channel, TRPV4, and its importance in cartilage health. Degenerative joint conditions, such as osteoarthritis, are associated with pro-inflammatory signalling which causes degradation of the articular cartilage leading to … [more]
Many congratulations to Jinshuai, who successfully defended his PhD thesis in November, 2020. Jinshuai's thesis is titled "Synthesis and Optoelectronic Study of Geometry-Confined Lead Halide Perovskite Monocrystalline Thin Films". Jinshuai was funded by a CSC-QMUL PhD studentship and was supervised by Dr Lei Su and Prof Martin Knight. During his PhD, … [more]
The EU Commission have published a report on some of the research into cancer mechanobiology taking place in the Bioengineering Division within School of Engineering and Materials Science at QMUL. The report entitled 'Mechanobiology of METAstatic and DORMant cancer cells in bone marrow lesions', describes research conducted by Dr Verbruggen … [more]
Thanks to the new world of on-line events, the OOACT Network's sandpit – originally planned face to face in April this year – was run successfully in MS Teams from 29 September to 1 October. This interactive funding sandpit event provided a great opportunity for 32 senior academics and industrialists from across the UK to … [more]
This project funded by Queen Mary Innovations, will identify compounds for the treatment of a rare genetic disease, Jeune Syndrome, that disrupts skeletal formation. The disease is caused by mutation in the genes that regulate primary cilia – the tiny hair-like cellular structures that coordinate a variety of important signalling pathways. … [more]
Queen Mary researchers develop non-distructive single-crystal perovskite surface nanopatterning technologies25 September 2020
Single-crystalline perovskites are widely regarded as the future semiconductor materials and will be the next big wave in optoelectronics. To manufacture high-quality single-crystal perovskite optoelectronics, non-destructive surface nanopatterning technologies are required. Conventional photolithography-based nanopatterning methods cannot be used due to perovskite’s sensitivity to high temperature and solvents. Researchers at … [more]
QMUL has launched a new e-symposia series with research presentations related to organ-on-a-chip and other predictive in vitro models. The symposia are part of the UK Organ-on-a-chip Technologies Network which is funded via the Technology Touching Life scheme and directed by Prof Hazel Screen and Prof Martin Knight, both bioengineers … [more]
The Centre for Predictive in vitro Models (CPM) was officially launched on Wednesday 22nd with an successful virtual symposium. More than 330 registrated attendees joined in to celebrate the occasion, listening to talks and participating in stimulating discussions. Introductory words from the vice principal for the faculty of Science and Engineering, … [more]
The Queen Mary & Emulate Organs-on-Chips Centre is involved in a new initiative to use miniaturised living systems with human cells, known as organ-chips, in the fight against COVID-19. As part of this initiative, Emulate, Inc have donated blank Organ-Chips to the UK Organ-on-a-chip Technologies Network to support the development of … [more]
New state-of-the-art microscope to support research in the School of Engineering and Materials Science20 May 2020
Queen Mary underlined its strong commitment to the ongoing research in SEMS by investing into a £375K state-of-the-art super-resolution spinning disc microscope. Microscopy imaging approaches have long been a major research strength within the school, facilitating new collaborative links and supporting significant research funding and outputs in leading journals. The … [more]
The Queen Mary + Emulate Organs-on-Chips Centre is supporting a new initiative to use predictive in vitro models, also known as organ-chips, in the fight against COVID-19. Emulate, Inc is participating in the Organs-on-Chips against COVID-19 initiative (#OoCovid) and donating a set of Organ-Chips to the UK Organ-on-a-Chip Technologies Network which … [more]
Cobalt ions from some orthopaedic implants induce a dose-dependent cytotoxic and pro-inflammatory response. However, this new paper from Prof Knight and Prof Shelton's groups shows that low levels of cobalt are in fact beneficial and block pro-inflammatory signalling in cartilage. The authors showed that sub-toxic levels of cobalt (50uM) prevented … [more]
On 29 January, Organ-on-a-Chip Technologies Network scientists and engineers swapped their lab coats for aprons, to run the Organ on a Chip shop at the hugely popular Science Museum Lates in London. This Lates night celebrated the launch of the Museum’s new Medicine Galleries and attracted over 4,000 visitors. Promoted as … [more]
During his time as Professor of Medical Engineering at Queen Mary University of London, Prof Bader was also a part-time visiting professor in the Technical University of Eindhoven (Tu/E). There he worked closely with Prof Cees Oomens in the area of pressure ulcers as well as continuing his work … [more]
A new research centre which aims to revolutionise medical research and drug development using microengineered Organs-on-Chips has opened at Queen Mary University of London. Organs-on-Chips contain tiny hollow channels lined by living human cells that recreate the microenvironment experienced by cells within the human body. As miniaturised living systems with … [more]
Profs Knight and Screen were recently interviewed for a news article on organ-on-a-chip which has just been published in the magazine Engineering & Technology. The article explored the potential of organ-on-a-chip technology for testing the safety and efficacy of new pharmaceuticals and the impact on conventional animal testing. [more]
Clare Thompson and Megan Mcfie have helped write a chapter on primary cilia mechanobiology in an excellent new book entitled Mechanobiology : From Molecular Sensing to Disease. The chapter reviews the role of primary cilia in mechanosignalling in a variety of tissues and the effect of mechanical forces on cilia structure … [more]
Science Museum Lates: Medicine, 18.45 - 22.00, Wednesday 29 January 2020 (over -18s only) Organ on a Chip Shop - a Lates highlight! Organ-on a-Chip Technologies Network members from across the UK will be running the shop at this hugely popular event. Based on the concept of a fish & chip takeaway, this 'make … [more]
Several members of the Knight group have been at the excellent International Conference on Cilia, Flagella and Centrosomes organised by the French and UK Cilia Networks. On the first day Megan gave a fantastic presentation on her PhD project screening >1700 compounds for effects on primary cilia structure as part of … [more]
Congratulations to Megan and Hudair who both won prizes for their PhD research posters at the Industrial Liaison Forum organised by the School of Engineering and Materials Science. Both Megan and Hudair are part of Knight’s research group. Megan’s PhD is co-supervised by Dr Cleo Bishop at the … [more]
At the SEMS annual research led Industrial Liaison Forum, there was a research showcase from 80 of our current PhD students. The panel of judges this year were made up a wide range of academics from each division. They decided after a careful deliberation to award the following prizes: 1st Prize: … [more]
Congratulations to Su Fu who passed his PhD on the effect of mechanical forces on cartilage inflammation22 October 2019
Su Fu has passed his PhD entitled: 'The role of primary cilia in the anti-inflammatory effects of mechanical loading in cartilage'. Su was supervised by Prof Martin Knight and funded by a grant from the China Scholarship Council. He has already published one very good paper in the journal Osteoarthritis … [more]
New research published in Nature Communications, reveals how the location of the protein Polycystin-2 (PC2) on primary cilia regulates Polycystic Kidney Disease.16 September 2019
Autosomal Dominant Polycystic Kidney Disease (ADPKD) is a common yet devastating disease. It is a heritable disorder characterised by development of kidney cysts which slowly overtake the kidney’s structure, destroying its function, and often resulting complete kidney failure by the of age 60. ADPKD affects as many as 1 in 400 people … [more]
Bioengineers and Cancer Biologists develop a complex in vitro model of the human tumour - new Ideaslab video6 September 2019
Bioengineers are trying to grow human organs, so could growing human tumours help us to understand the microenvironment in which they spread – and how the immune system might be persuaded to attack them? Frances Balkwill explains in this new Ideaslab video for the World Economic Forum how our research at … [more]
Queen Mary Researchers have been awarded funding for three separate biomechanics and mechanobiology pump priming projects. Professors Hazel Screen and David Lee of the School of Engineering and Materials Science were awarded funding from the OAtech+ Network to develop projects relating to mechanobiology aimed at targeting the chronic, degenerative disease … [more]
Our paper entitled 'Transglutaminase-2 Mediates the Biomechanical Properties of the Colorectal Cancer Tissue Microenvironment that Contribute to Disease Progression' has been accepted in the journal Cancers. This paper was led by Dr Nick Peake and funded by our grant from Bowel & Cancer Research. Biomechanics analysis was provided by my PDRA, … [more]
Queen Mary hosted the UK Organ-on-a-chip Technologies Network for a special Learning and Collaborative Event. The network has over 450 members from 50+ academic institutions with over 50 partners from industry and other stake holders. The network event included a programme of research talks, discussion groups, industry demonstrations and special training sessions on … [more]
Exercise helps to prevent the degradation of cartilage caused by osteoarthritis, according to a new study from Queen Mary University of London. The researchers show for the first time how mechanical forces experienced by cells in joints during exercise prevent cartilage degradation by suppressing the action of inflammatory molecules which … [more]
A new paper from Prof Martin Knight's group examines the use of two different methods of providing mechanical ventilation to premature babies. The study led by Prof Anne Greenough and colleagues at King's College London, explores the effect of lung inflation volume on inflammation within the alveolar epithelial cells. The … [more]
‘Neuron Pod’ - a striking 23-metre long and 10-metre high free-standing structure – has opened its doors as an informal science learning centre at Queen Mary University of London’s Whitechapel campus. The launch was marked by a visit from children from Christ Church Primary School, Brick Lane, who took part … [more]
The UK Organ-on-a-Chip Network official launch took place last week. On the 6th of September there was a conference day, held in conjunction with the BioMedEng18 Conference, taking place at Imperial College London and followed by a launch day at Queen Mary’s Graduate Centre on Friday the 7th. This … [more]
Mechanical regulation of primary cilia in tendon cells suggests a novel feedback mechanism controlling tendon health and mechanosensitivity.23 July 2018
New research from Prof Martin Knight and Prof Hazel Screen's group at Queen Mary University of London reveals a novel feedback mechanism regulating tendon health and homeostasis during mechanical loading. The study published in Scientific Reports investigates primary cilia in tendon cells subject to physiological and pathological mechanical loading. Primary … [more]
I attended the excellent eCM Cartilage conference in Davos Switzerland and presented Su Fu's PhD work on the role of primary cilia in the anti-inflammatory effects of mechanical loading. Lots of interesting talks. Also managed to get in some walking high in the Alps! [more]
Queen Mary University of London has been awarded funding to lead a UK network in the development of ‘Organ-on-a-chip’ devices. The network will be part of a major new Research Councils UK (RCUK) funding venture, Technology Touching Life , which aims to foster interdisciplinary research into innovative technology in the health … [more]
Congratulations to Dan Rowson who passed his PhD viva. Dan's PhD thesis investigated the effect of mechanical stimulation on tendon cells in order to better understand the process of tendon injury. In particular, Dan investigated the effect of mechanical forces on tiny hair-like cellular structures structures, known as primary cilia. … [more]
A new paper published in the journal Cancer Discovery, by Prof Martin Knight and colleagues describes, for the first time, an evolving human metastatic microenvironment. The work led by Prof Fran Balkwill measures gene expression, matrisome proteomics, cytokine and chemokine levels, cellularity, extracellular matrix organization, and relates this to changes … [more]
Congratulations to Dr Luming Zhao, who has been awarded a two-year H2020 Marie Curie Fellowship (€200k), supervised by Dr Lei Su, Prof Martin Knight and Dr Manoj Ramachandran. He will develop novel compact optical fibre lasers for clinical imaging and laser surgery. [more]
When we loose cartilage tissue in the knee joint and suffer from knee pain, the joint function is impaired and the patient may need a total knee replacement surgery. The procedure involves removal of the damaged and diseased joint and replacement with an artificial joint. Most patients who undergo knee … [more]
Dr Stefaan Verbruggen has recently joined the bioengineering group at Queen Mary on a Marie Sklodowska-Curie Actions Individual Fellowship. Stefaan is working in Prof Martin Knight's research group investigating the role of mechanical forces in bone cancer and the involvement of primary cilia. Stefaan's project is entitled 'META-DORM: Mechanobiology of … [more]
Prof Martin Knight presented an invited talk at a special primary cilia session at the Back2Back meeting organised by Prof Sally Roberts. This excellent research meeting at the Robert Jones & Agnus Hunt Orthopaedic Hospital in Oswestry focused on back pain and intervertebral disc with a special cilia afternoon session … [more]
A new paper from Prof Knight's group shows how expansion of cartilage cells for tissue engineering disrupts primary cilia expression inhibiting associated cellular signalling. Primary cilia are specialised, hair-like structures found singly in the majority of cell types where they coordinate a variety of cell processes important for tissue development, … [more]
Dr Gupta and Prof Knight have just published exciting results which cast light on how cartilage is able to withstand the demanding mechanical environment of the joint and may eventually help to explain why cartilage breaks down with ageing or arthritis. Collagen changes its crystallinity in response to physical forces, … [more]
Martin Knight's research group (and family!) took on Hazel Screen's research group (and extras) for a netball match. Despite heroic efforts from our team Hazel's group won (just)!
Supercapacitors promise recharging of phones and other devices in seconds and minutes as opposed to hours for batteries. But current technologies are not usually flexible, have insufficient capacities, and for many their performance quickly degrades with charging cycles. Researchers at Queen Mary University of London (QMUL) and the University of … [more]
Two students working in Prof Knight's research group received grant funding to continue their individual research projects over the summer. The two students have been doing research project as part of their Intercalated BSc in Biomedical Engineering and Clinical Materials. The funding from the Rod Flowers Scholarship scheme will enable … [more]
In November 2016, we celebrated 25 years since the start of the Interdisciplinary Research Centre (IRC) in Biomedical Materials at Queen Mary University of London. The IRC which was set up by Prof Bill Bonfield delivering high quality research including the development of bone graft substitute materials. Prof Knight was one of … [more]
Prof Knight presented an invited lecture on super resolution microscopy at this meeting on Primary Cilia Dyskinesia in Valencia. I also had a fantastic bike ride down the coast to see flamingoes!
Prof Knight's group has published a new paper which shows how a novel bone graft substitute biomaterial promotes osteogenic differentiation of stem cells associated with regulation of primary cilia structure and TGFB signalling. The paper published in the journal Acta Biomateriala is based on the work of PhD student, Melis … [more]
|UK Cilia Network meeting. Several members of the group attended this excellent meeting in Oxford. Sondos presented her 3rd year Biomedical Engineering research project on FGF and cilia, James and Su presented their PhD work looking at changes in cilia expression during chondrocyte passage and effect of mechanical loading on cilia mediated inflammatory signalling. (March 2017)|
EPSRC OAtech network plus - I have been appointed as the theme lead for Biomechanics and mechanobiology of OA and the relationship to clinical measurements. More information to follow.
Two papers published on primary cilia in alkapatonuria - We have just had two papers published on changes in chondrocyte primary cilia structure and function in the rare genetic disease, alkaptonuria. These papers in Journal of Cellular Phsiology in collaboration with Prof Antucci (University of Sienna), show how alkaptonuria causes changes in primary cilia length, trafficking and proteome associated with disregulation of hedgehog signalling.
Bioengineering in Ireland23 - I attended the excellent Bioengineering in Ireland conference where I gave a keynote on mechanobiology and primary cilia. The conference has a really nice atmosphere with great presentations from post docs and PhD students and an awesome social program!
Dan Rowson's paper on cilia in tendon makes front page!- Dan's paper on tendon primary cilia and the effect of mechanical loading is featured on the front cover of the Journal of Orthopaedics Research.
|Royal College of Surgeons (RCS) Grant - Congratulations to Ahmed Ali who has been awarded a small grant from the RCS to work on a project in my group on primary cilia and osteoarthritis. Ahmed is currently studying for an intercalated BSc in Biomedical Engineering and Clinical Materials here at Queen Mary. The grant will support his individual research project on the interaction between mechanical loading and inflammation. (December 2016)|
|Melis Dalbay passes her PhD - Congratulations to Dr Melis Dalbay who has passed her PhD. Melis worked on primary cilia in mesenchymal stem cells. In particular she has studied the effect of physical and chemical manipulation of primary cilia structure and how this influences adipogenic and osteogenic differentiation. She has already published one paper in the journal Stem Cells on IGF signalling in adipogenic differentiation with two further papers on the way. (November 2016)|
Cilia 2016 Conference - Several of the team attended the Cilia 2016 conference in Amsterdam. Clare Thompson presented some of exciting new data on the role of polycystin suggesting a new mechanisms of primary cilia mediated mechanosignalling. Melis Dalbay presented work from her PhD demonstrating biomaterial regulation of primary cilia elongation and how this control TGFB mediated osteogenic differentiation of mesenchymal stem cells. (October 2016)
Our new public engagement Interactive goes live at the Centre of the Cell - Following the successful stand at the Royal Society Summer Exhibition, the online app has now been installed in the main pod at the Centre of the Cell (Queen Mary's interactive science centre). The interactive entitled 'tumour take down' is part of the CANBUILD project which aims to bioengineering a human tumour microenvironment.
British Society for Matrix Biology (BSMB) meeting - 'The art of communication: Signalling cascades in mechanotransduction' (Cardiff, September). I presented a keynote lecture on some of our work looking at primary cilia mechanobiology. Dr Clare Thompson gave an excellent presentation on new data suggesting a totally new paradigm of primary cilia mediated mechanosignalling and Dr Rowson presented his latest PhD research on the effect of mechanical loading on tendon cell primary cilia.
Research group away day joining up with Prof Hazel Screen's group for GoApe and Rounders!
|The EU funded CanBuild project to bioengineer a human tumour has been presenting at the Royal Society Summer Exhibition this week. As part of the multidisciplinary team, I am working with post doc, Dr Robin Delaine-Smith, on understanding the role of biomechanics in regulating how the tumours grow and spread. See QM press release and write up in the Guardian.|
Pint of Science - I presented a public talk on my research as part of the Pint of Science festival on 23rd May at Redchurch Brewery! The talk in conjunction with Prof Julia Shelton, focussed on aspects of bioengineering related to the hip joint and the problem of arthritis which affects 8 million people in the UK. Full details at http://pintofscience.co.uk/event/from-hip-cells-to-implants
David Lee, Me and Dan Bader (David and Dan were my PhD supervisors at the IRC in Biomedical Materials, QMUL!
New biomechanics paper accepted: Experimental validation of a flat punch indentation methodology calibrated against unconfined compression tests for determination of soft tissue biomechanics RM Delaine-Smith, S Burney, FR Balkwill, MM Knight - Journal of the Mechanical Behaviour of Biological Materials. This work for this paper was done by Robin Delaine-Smith as part of the CANBUILD project funded by the ERC which aims to bioengineer a human tumour. The first stage of this process has been a full 'deconstruction' of the natural tumour microenvironment including biomechanics, proteomics and transcriptomics. The paper was also supported by an undergraduate medical engineering student, Saba Burney, who performed some of the biomechanical testing (February 2016)
And another one... Congratulations to Kristina and Zhao who's work on the effect of LifeAct-GFP on chondrocyte biomechanics and bleb formation has just been accepted for publication in the Journal of Biomechanics.
3 new journal papers accepted this week from my group:
- Clare L. Thompson; Riana Patel; Terri-Ann N. Kelly.; Angus K. T. Wann; Clark T. Hung; J. Paul Chapple; Martin M. Knight. Arthritis Research & Therapy. Hedgehog signalling does not stimulate cartilage catabolism and is inhibited by Interleukin-1β
- Kristina Sliogeryte; Lorenzo Botto; David A. Lee and Martin M. Knight. Osteoarthritis and Cartilage. Chondrocyte dedifferentiation increases cell stiffness by strengthening membrane-actin adhesion
- Tan A, VandenBerg C, Attur M, Abramson S, Knight MM, Bulinski J, Ateshian G, Cook JL, Hung C. Arthritis Research & Therapy 2015, 17 :361 (2015) Cytokine preconditioning of engineered cartilage provides protection against interleukin-1 insult
New Post Doc Position. There is a new post doc position currently being advertised to look at primary cilia electrophysiology using scanning ion conductance microscopy. The post is funded by the Wellcome Trust seed grant awarded to Dr Pavel Novak in the School of Engineering and Materials Science at QMUL. Application details here: https://webapps2.is.qmul.ac.uk/jobs/job.action?jobRef=QMUL7630 (December 2015)
Visit to Irish Cilia Network meeting and Trinity Centre for Bioengineering. I visited Dublin to give a seminar at the Trinity Centre for Bioengineering and then a keynote lecture at the Irish Cilia Network meeting. It was fantastic to meet David Hoey, Daniel Kelly, Oliver Blacque, Cairan Morrison, Catriona Lally and Paula Murphy and their research teams to hear about some of the great primary cilia research going on in Ireland and discuss opportunities for collaboration. (November 2015)
New PhD student, Su Fu , joins the group. We welcome Su Fu who joins my research group funded on a China Scholarship Council PhD project for 4 years. Su's project will examine the role of mechanically induced changes in primary cilia in regulating the inflammatory signalling cascade. Initial studies will focus on articular chondrocytes building on previous studies from our group. (More details) (November 2015).
Congratulations to Zhao Wang who passed his PhD viva. Zhao's PhD, which was funded via the China Scholarship Council, examined the influence of osmotic challenge on chondrocyte biomechanics. Zhao has already published two papers in the journal, 'OA and Cartilage' with a further paper currently in revision with the Journal of Biomechanics. The work involved micropipette aspiration for measurement of cellular mechanical properties coupled with confocal analysis of actin dynamics using LifeACT-GFP (see images below). (November 2015)
Media coverage following our latest paper on the effects of lithium on cilia and hedgehog signalling. Clare Thompson's paper from our group was published in FASEB J and shows how lithium chloride causes elongation of the primary cilia which results in reduced sensitivity of the hedgehog signalling pathway. The findings demonstrate exciting potential of ciliotherapy in which the structure of the cilium is modulated to control hedgehog signalling which is involved in osteoarthrititis and other pathologies. see press release and subsequent media coverage including Science Newsline, Follow News and Digital News World (November 2014)
Sheetal Inamdar wins poster prize at Institute of Bioengineering Launch Event. Sheetal won the prize for her poster describing her PhD research on the nanomechanics of collagen fibrils within cartilage and the effect of disruption of the chondrocyte primary cilia. The Institute of Bioengineering launch (photo below) was attended by over 200 people who came to mark the launch of this ambitious cross-Faculty initiative and to hear about QMUL’s diverse scientific research portfolio in bioengineering. (October 2015)
Wellcome Trust Seed Award funded. “Mapping mechanotransduction mediated by primary cilia at nanoscale “. This grant awarded to Dr Pavel Novak will combine the latest technology in scanning probe microscopy and nanopipette techniques with confocal microscopy, electrophysiology, and mechano-biology to break the existing resolution barrier in direct stimulation and recording of channel and receptor activity along the fine structure of primary cilia. The technique will be used to gather data on the distribution of mechano-chemical receptors in primary cilia to support future research proposals aimed at understanding the reorganisation of mechanotransduction in ciliated cells in disease. I am the named collaborator on this grant which supports the groups work on cilia structure-function relationships. (September 2015)
Paper published on chondro-protective effects of lithium. Our paper was published in Journal of Orthopaedic Research describing how dietry lithium chloride is not catabolic in vivo and also prevents the degradation of articular cartilage in response to the inflammatory cytokine, IL-1B. The work was led by Dr Clare Thompson with support from two Medical Engineering undergraduate students (Anna Verone and Habiba Jasmin) with grant funding from the AO Foundation in collaboration with Prof Tony Poole in New Zealand. The paper has attracted some media interest following a QM press release
Cynthia Jensen visits from Auckland, New Zealand. We were delighted to have Prof Cynthia Jensen visit our group. Cynthia was involved in much of the pioneering work on primary cilia in connective tissue and hosted my previous research visits to University of Auckland in 1998 and 2006. While Cynthia was visitng us in we had talks from some of the cilia research team (from left: Me, Dan Rowson, Sheetal Inamdar, Melis Dalbay, Robin Delaine-Smith, Riana Patel and Cynthia Jensen). (July 2015)
I love Super Resolution microscopy of primary cilia! Just had a lovely time taking images of primary cilia in human chondrocytes. The following image obtained using structural illumination with 3D reconstruction - red is acetylated a-tubulin, green is pericentrin. This primary cilia has a length of approximately 2 microns. (June 2015)
Silvia Gambassi joins our group to examine the role of primary cilia alkaptonuria. Silvia is a visiting PhD student from Prof Annalisa Santucci's lab in the University of Sienna, Italy. Her work is investigating whether cartilage degeneration in alkaptonuria involves changes in primary cilia structure and function. She has recently taken this lovely confocal image of primary cilia in human chondrocytes with acetylated a-tubulin (red) and pericentrin (green).
Riana Patel wins Rod Flower Scholarship. Riana is a medical student doing an intercallated BSc in Biomedical Engineering at Queen Mary and has been working on her research project investigating the role of hedgehog signalling in cartilage pathology.The scholarship will allow Riana to work through the summer on a new project examine the role of de-differentiation on chondrocyte primary cilia. (April 2015)
Interview in EngineeringBecause. I was recently interviewed for the website Engineering Because about my career in medical engineering and mechanobiology. click here to see full article (April 2015)
Royal Society Mechanobiology meeting. I presented some of our research at this small invited research meeting. The programme included presentations from Don Ingber, Matt Dalby and Denis Discher's lab (April 2015)
New Cancer Biomechanics pump priming grant from QM Life Sciences Inistitute. The grant is entitled 'Characterising the therapeutic impact of immunoglobulins on tumour biomechanics' and is in collaboration with Dr Melania Capasso at Barts Cancer Institute. (April 2015)
New grant awarded by Bowel & Cancer Research. The grant, led by Dr Nick Peake (SEMS) and in collaboration with Dr Alex Mirnezami at the University of Southampton, will study the role of biomechanics in the progression of colon cancer. More details. (March 2015)
Congratulations to Kristina Sliogeryte who passed her PhD. Her project, supervised is entitled: 'The role of membrane-actin adhesion in regulating stem cell viscoelastic properties and blebability during differentiation?' (17th March 2015)
Launch of the new Primary Cilia research group web site. We have launched a new web site bringing together primary cilia research taking place at Queen Mary University of London. http://www.primarycilia.qmul.ac.uk/
Undergraduate 'Best Project Prizes' - My 3rd year Medical Engineering undergraduate student, Shyam Patel, won the prize for the best research project at the recent Industrial Affiliates Forum hosted by the School of Engineering and Materials Science. Shyam's project examines the effect of enzymatic degradation on the local strain fields and mechanical properties in articular cartilage. The project links with PhD student, Sheetal Inamdar, who is working with me and Dr Himadri Gupta on cartilage collagen nanomechanics assessed using small angle x-ray defraction.
My 4th year Medical Engineering group R&D project also won the best 4th year project prize for their work on testing of a novel vascular graft in collaboration with an industrial sponsor. (March 2015)
Cilia elongation controls stem cell adipogenesis - Paper published in Stem Cells. Our paper has just been published showing the adipogenic differentiation of human mesenchymal stem cells is mediated by primary cilia elongation and associated recruitment of insulin-like growth factor receptor (IGF-R) to the primary cilium. The paper is part of Melis Dalbay's PhD studies on the role of primary cilia in stem cell differentiation. Click here for full paper. See QMUL Press Release and associated write up in Science Daily, BioPharma-Reporter, diabetes.co.uk and MF Monitor (February 2015)
New PhD Studentships. I am currently advertising for a new PhD student to work on the role of polycystin in cartilage mechanobiology in health, aging and disease. The student will be trained in state-of-the-art techniques including super resolution microscopy and scanning ion conductance microscopy (SICM) enabling nanoscale visualisation of polycystin on primary cilia and electrophysiology of this possible mechanosensitive ion channel. This exciting new project will be co-supervised by Dr Pavel Novak (an expert on SICM) and Dr Cleo Bishop in the School of Medicine and Dentistry (an expert in primary cilia and ageing). [More infomation] (January 2015)
Our paper on cell mechanics and osmotic challenge published in OA and Cart. Congratulations to Zhao Wang as his paper has just been published in OA and Cart. The study examined the influence of rate of hypoosmotic challenge on chondrocyte morphology, actin organisation and regulatory volume decrease. The study used micropipette aspiration to reveal how these factors induced changes in cellular mechanics. click here to view the paper (valid until 11th March)
Our Bionic Man interactive on the BBC. The BBC news recently ran a report on the skills gap in engineering and science and the need to encourage more students to study engineering at university. The BBC report showed a nice image of our 'Bionic Man' interactive that we have presented at various science festivals encourageing school children to consider careers in bioengineering. (January 2015)
Farewell to Angus. After six years as a post doc in my group, Dr Angus Wann is leaving to take up a personal Fellowship at the Kennedy Institute in Oxford. Angus is an outstanding scientist who has made a major contribution to the success of our group and the field of primary cilia research. He has published some excellent papers and helped write three successful project grants. He has been an enthusiastic team player and we will all miss him greatly but I am sure the collaboration and friendship will continue. Good luck Angus! (December 2014)
External Examiner Appointment. Prof Knight has recently been appointed as the external examiner for the Biomedical Engineering (BEng and MEng) undergraduate degree programmes at Imperial College, London. This degree programme at Imperial and the medical engineering programme at QMUL represent the two largest undergraduate bioengineering degree programmes in the UK. (December 2014)
Cilia 2014 Conference. Our group presented two abstracts at the Cilia 2014 meeting in Paris. Melis Dalbay gave an excellent oral presentation on the role of primary cilia elongation in adipogenic differentiation. Dr Clare Thompson presented some of her latest data on the effect of lithium on primary cilia structure and hedgehog signalling in chondrocytes. Click here for full programme. (November 2014).
First Super Resolution Microscopy images of Primary Cilia. We were excited to take our first images of primary cilia using the new super resolution microscope purchased by the Institute of Bioengineering. The images (below) were captured using structural illumination microscopy (SIM) and show the location of ARL13b (green) around the perifery of the primary cilium (red - acetylated alpha tubulin). To our knowledge this is the first time that ARL13b has been imaged with sufficient resolution to suggest its membrane location (October 2014).
Holidays - Happy memories of the French Alps!
F1000 prize for work on primary cilia length and hedgehog signalling. ClareThompson's work on the relationship between chondrocyte primary cilia length and hedgehog signalling won the F1000 prize based on the poster which I presented at the eCM conference ‘Cartilage & Disc: Repair and Regeneration’. More details here. (July 2014)
Bioengineering the Bionic Man. Martin gave a public presentation at the Barts and Queen Mary Science Fair describing some of the bioengineering research that his group and others are doing to help treat medical problems such as meniscal tears, ruptured ligaments and arthrtitis. (July 2014)
Martin presents at eCM conference. We had three posters and an oral presentation at the cartilage repair meeting in Davos, Switzerland. I presented our work on chondrocyte dedifferentiation and changes in primary cilia and hedghog signalling as well as two posters on the effects of lithium on cilia and cartilage degradation and one on the role of cilia in IL1 NFkB signalling. Thanks to Angus and Clare who have done much of this work funded through grants from the ARUK and AO Foundation.
I also got to spend some time walking in the fantastic Swiss Alps. (June 2014)
Martin chairs Biomaterials session at Prof Priestley's retirement symposium. I attended the symposium for Prof John Priestley who is retiring from QMUL. He and I have collaborated on neural mechanobiology and we jointly supervised, along with Prof Adina Michael-Titus, an excellent BBSRC PhD student (Stacy Gladman).
Medical Engineering prize - student blog. Anna Varone has written a nice blog on the QMUL website describing how she won the IMechE prize for best medical engineering student project. Her project (part of her undergraduate degree in Medical Engineering) looked at the effect of lithium on cartilage degradation in the presence of inflammatory cytokines. The work is part of a grant from the AO Foundation in collaboration with Prof Tony Poole and with Dr Clare Thompson as the post doc. We will present this at the ECM Cartilage meeting next week and hope to submit as a full journal paper shortly. Anna is off to Aberdeen to start a PhD linked with Oxford Biomaterials and working on silk conduits for neuro-regeneration. (June 2014)
Research Group Away Day We joined with Dr Hazel Screen's research group for an away day to Box Hill in Surrey. Excellent picnic and rounders but poor Zhao painfully dislocated his shoulder in an epic attempt to win the relay race for team Knight. Fortunately he is now recovering well. (June 2014)
New primary cilia paper published. Our paper on the role of the primary cilium in NFKB signalling induced by IL-1 has been published in Journal of Cellular Signalling. Dr Angus Wann conducted the research as part of his post doc funded by a grant from the ARUK. (May 2014)
Urine Superpowers documentary wins award Last year I was filmed as part of a documentary on urine. This followed my paper on fluid mechanics analysis of the spiral pattern made by urine as it leaves the eliptical shaped male urethra and its potential diagnostic use (PLOSone 2012). The documetary has recently won an award (Le Trophy d'Or) and will soon be shown on French TV. (May 2014)
Four abstracts accepted at ECM Cartilage conference. Delighted to hear we have three posters and one oral presentation at the forthcoming European Cells and Materials conference on Cartilage Repair in Davos, Switzerland. The papers relate to the primary cilia work of Dr Angus Wann and Dr Clare Thompson and also a nice study on the chondroprotective nature of lithium chloride in collaboration with Tony Poole. (April 2014)
Our new Cilia paper published in Journal of Cellular Signalling. Dr Angus Wann has just published his paper entitled: The primary cilium influences interleukin-1β-induced NFκB signalling by regulating IKK activity. The paper stems from studies funded by our ARUK grant on the role of cilia in inflammatory arthritis. (April 2014)
Visit to Finland for PhD viva. I visited Finland to be the official opposer for Hannu Karjalainen's PhD defence at the University of Eastern Finland, Kuopio. Hannu (2nd from left) was supervised by Prof Mikko Lammi (left) and his thesis was entitled: 'Transcriptional Responses of Chondrocytic and Osteoblastic Cells to Physical Forces of Hydrostatic and Osmotic Pressure, Mechanical Stretching, and Low Intensity Pulsed Ultrasound'. Whilst there I was treated extremely well including a chance at ice hole fishing and a drive over the frozen lake (pictured) as well as the fantastic 'after party' (February 2014)
IMechE Best Medical Engineering student project prize Two undergraduate medical engineering students working in my group, Anna Varone and Habiba Yasmin, won the Vicon prize for the best Medical Engineering project at the 26th annual biomedical engineering student project competition organised by the Institute of Mechanical Engineers. Their projects have examined the effect of lithium chloride on the cartilage degradation in the presence of the inflammatory cytokine IL-1B. The pair are supervised by me but have also had great support from Dr Clare Thompson, who is a post doc on our AO Foundation grant looking at the effect of lithium on cartilage and primary cilia. (February 2014)
Three posters at the Keystone Symposia on Cilia. Dr Angus Wann, Dr Clare Thompson and Melis Dalbay will all be presenting some of their latest research at the prostigeous internation Keystone Sympoisa on Cilia, Development and Human Disease being held in Calafornia. (February 2014)
New QM research film on the CANBUILD grant. The college has produced a new short film highlighting the research taking place in the CANBUILD project funded by an ERC grant awarded to Prof Balkwill. The aim of the project is to engineer a human tumor microenvironment in vitro. Dr Robin Delaine-Smith is the post doc in my group working on measuring the biomechanics of omental tumours with a view to using the mechanical properties to drive tumorogenesis. (January 2014)
Watch the film on youtube: http://www.youtube.com/watch?v=V61eD9oJD48
Cilia paper accepted in Osteoarthritis and Cartilage. The paper is based on Clare Thompson's PhD research and examines the influence of mechanical loading on primary cilia structure and associated hedgehog signalling. (January 2014)
Media coverage following our paper on cilia and topography induced differentiation. Following publication of Rebecca McMurrey's paper in Scientific Reports, Queen Mary issued a press release which led to a write up in The Scientist Magazine - Surface Texture Influences Differentiation . (December 2013)
Christmas Party and Sports. The Knight research group joined up with Dr Hazel Screen's group for a rather strange sporting event - Netball/hangman - which required a combination of physical and mental dexterity! This was followed by Christmas Dinner which required neither. (December 2013)
Three new primary cilia journal papers accepted from the group.The Knight research group have just had three new primary cilia journal papers accepted for publication. The first study from Dr Angus Wann is to be published in the journal Cilia and demonstrates the interaction of HIF signalling and primary cilia in response to inflammatory cytokines. Dr Jerome Irianto in collaboration with Prof Rosa Serra, had his paper accepted by Journal of Biomechanics on the effect of depletion of primary cilia on the mechanical properties of cartilage. Finally Dr Rebecca McMurrey, Dr Angus Wann and Dr Clare Thompson have had a paper accepted in Scientific Reports. Their study unravelled the role of primary cilia elongation in mediating stem cell wnt signalling and differentiation in response to micro grooved topography. (December 2013).
Our paper on development of a spinning-disk super resolution microscope published in PlosOne. Dr Neveen Hosny has published our paper on the development of a super resolution microscope system that she built during her post doc position in my group funded by an EPSRC discipline bridging grant in collaboration with Dr Ann Wheeler. The paper in Plos One and the associated Queen Mary press release has been picked up by various science websites and is to be written into a full article for The Biologist magazine. (October 2013). http://www.qmul.ac.uk/media/news/items/se/115135.html
Our paper has been published on why cat urine doesn't cause encrustation of urological stents. Recent research from MSc Bioengineering student, Manar Shafat, has just been published exploring the difference between cat urine and human urine in terms of the rate of encrustation of urological stents. Manar's work showed that the differences in the inorganic chemical composition of human and cat urine is not responsible for the difference in encrustation suggesting it is the different organic components that prevent encrustation in cats. See full news report: 'Can eating Cat food cause urinary problems'
Dr Tony Poole visits and speaks at London Cilia meeting. Tony Poole from University of Otago (New Zealand), one of our long standing collaborators, visited our group on 2nd October and spoke at a meeting on primary cilia researcher that we organised here at Queen Mary. The meeting included Prof Cheetam (UCL Institute of Opthalmology), Dr Mittchinson & Prof Phil Beales (UCL Institute of Child Health), Dr Paul Chapple (QMUL WIlliam Harvey Research Institute) and members of these groups. (October 2013).
Clare Thompson passes her PhD viva. Many congratulations to Clare whos PhD examined the influence of mechanical and thermal stress on chondrocyte primary cilia and associated hedgehog signalling. Clare is continuing in the group as a post doc working on an AO Foundation grant looking at the effect of lithium on chondrocyte primary cilia and cartilage degradation. (September 2013).
MRC Grant Successful. We now have confirmation that the MRC will fund our grant entitled: 'Osteoarthritis may be treated as an environmental ciliopathy'. The grant tests the hypothesis that pathological cues such as mechanical loading and inflammatory cytokines, modulate primary cilia structure and function leading to the onset and progression of arthritis. The grant is in collaboration with Prof Phil Beales and Dr Hannah Mitchinson (UCL) and Dr Paul Chapple (QMUL) and Mr Manoj Ramachandran (Barts & The London NHS). (September 2013)
Melis Dalbay and Kristina Sliogeryte present posters at the 7th UK Mesenchymal Stem Cell Meeting. Melis presented her PhD research in a poster entitled 'The role of primary cilia elongation in human mesenchymal stem cell differentiation'. Kristina's poster was entitled 'Chondrogenic differentiation regulates cell mechanics and migration by strengthening the interaction between the cell membrane and cortical actin'. Steven Thorpe from our research group also attended the meeting. (September 2013)
Jerome Irianto publishes a paper from his PhD describing a method for quantifying chromatin condensation from confocal microscopy images. Jerome, who is now doing a post doc in the USA, has had his paper accepted in Medical Engineering and Physics. The paper is entitled 'Quantification of Chromatin Condensation Level by Image Processing'. (September 2013)
Clare Thompson wins prize for primary cilia presentation at the British Society for Matrix Biology (BSMB) meeting in Cardiff. Clare's presentation was entitled: 'Primary cilia disassembly down regulates mechanosensitive hedgehog signalling - a feedback mechanism controlling ADAMTS5 expression in chondrocytes'. The theme of the meeting was 'Under pressure: the cell's response' with presentations describing the response of cartilage, tendon and other tissues to mechanical loading. Prof Knight was also at the meeting along with Dr Angus Wann who presented a couple of posters on his primary cilia research. We all joined Dr Hazel Screen and some of her research group for a day walking in the Black Mountains. (September 2013)
Priyanka Pravincumar (recent PhD graduate from the group) has got a post doc position at the AO foundation in Switzerland. Congratulations to Priyanka who's PhD thesis concerned the mechanical properties of chondrocytes and the role of actin dynamics. She published three nice papers in PLOSone, Biophysical Journal and Human Molecular Genetics. We look forward to visiting her in the Swiss Alps! (August 2013).
Research team wins at Rounders! Our research group joined up with Hazel Screen's group for an awayday picnic and rounders match in the Chilterns. We won the rounders but lost out in the relay race! All returned refreshed and a little sunburnt. (July 2013)
Robin Delain-Smith joins the group. Robin is part of the CANBUILD project funded by an ERC grant to Prof Fran Balkwell. The project aims to develop an in vitro tumour micro environment using tissue engineering techniques. Robin will be involved in the mechanical characterisation of the natural tumour environment, providing data for the development of tissue engineered scaffolds. He will also be involved in examining the response of adipogenic and cancer stem cells within the engineered scaffolds. (July 2013)
Clare Thompson Runner up in Poster Competition. Clare Thompson presented her PhD research at the William Harvey annual review at QMUL and was awarded the runner up prize for the poster competition. Her PhD research examines the influence of mechanical loading on chondrocyte primary cilia and hedgehog signalling and the implications for development of osteoarthritis. She is supervised by Martin Knight (SEMS) and Paul Chapple (William Harvey) and has just submitted her PhD thesis. (June 2013)
Farewell to Dr Jerome Irianto who leaves the group for a new post doc position in the USA. Jerome completed his MEng in Medical Engineering at QMUL and then went on to do a PhD supervised by Prof Knight and Prof Lee. He then continued his studies as a post doc working on the effect of osmotic and mechanical stimuli on nucleus chromatin condensation and cell mechanics. The group enjoyed a day out rowing at Henley on Thames as part of Jerome's farewell celebrations.
Research group outing - rowing at Henley on Thames. (May 2013)