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Queen Mary University of LondonQueen Mary University of London
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School of Engineering and Materials Science
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PhD Thesis: The Preperation and Characterisation of Porous Degradable Chitosan Structure for Tissue Engineering

Author: HSU, Hung-Liang

Year: 2010

Supervisor(s): Ray Smith

The development of degradable, porous scaffold structure is currently a major area of research in the tissue engineering and drug-release control field.

This thesis reports on research conducted into the development of cross-linked Chitosan porous structures. Glutaraldehyde and Genipin have been used as cross-linking agents. Chitosan has been cross-linked with varying amounts of cross-linking agents, and the effect of initial concentration of Chitosan has also been studied. Several methods of freeze drying have been investigated. It has been shown that the specific surface area of the porous structures can be manipulated by changing the initial concentration of Chitosan and also by changing the method of freeze drying. The degradation rates of porous Chitosan can be controlled by the degree of both chemical cross-linking and physical cross-linking influenced by the freeze drying method. Relationship between thermal properties, physical properties, enzymatic properties and contact angles with various type of cross-linking agent, degree of cross-linking and fabrication method are also analysed and discussed.

The porous, degradable Chitosan scaffold structure fabricated with 2 wt% initial concentration of gel-like Chitosan solution cross-linked by Genipin (molar ratio of GP to Chitosan is 0.25) frozen by electrical freezer showed the favourite conditions for the required degradation rate, thermal, physical properties of porous biodegradable scaffold.