Confocal and Super-resolution Microscopy Facility
Within the School of Engineering and Materials Science we have the following two confocal microscope systems with multi photon imaging capability, and a super resolution system purchased through the Institute of Bioengineering, as well as two standard epifluorescence microscopes and an incubator based microscope for live cell imaging, and Chiaro nanoindenter mounted with Leica DMi8 for measuring mechanical parameters of cells and materials.
Zeiss Super resolution LSM710 ELYRA PS.1
LSM 710 ELYRA PS.1 is a combination of an inverted laser scanning confocal microscopy 710 (LSM 710) with super resolution imaging microscopy ELYRA PS.1: photo activated localization microscopy (PALM)/Stochastic Optical Resolution Microscopy (STORM) and structured illumination microscopy (SIM). It is suitable for imaging targeting at 10-100nm resolution at single molecule level. In addition, Zen 2012 software, motorised X/Y stage, definite focus, TIRF mode make it capable of acquisition of tiling, multiple positions, time lapse, FRAP and FRET. It has a on stage environment chamber with a 35mm petri dish adaptor linked with a CO2/N2 controller for live cell imaging including hypoxic experiments. Furthermore, it comes with an offline data processing workstation.
Objectives:
- EC Plan-Neofluar10x/0.3 M27,
- EC Plan-Neofluar20x/0.5 M27,
- Plan-Apochromat 63x/1.4 Oil DIC M27,
- Plan-Apochromat 100x/1.46 Oil DIC M27
Lasers:
- LSM 710: Diode laser 405nm (30mW), Ar/ML 458/488/514nm (35mW), HeNe 543nm (1 mW), HeNe 633 nm (5mW)
- SIM/PALM: HR Diode 405nm (50mW), HR Diode 488nm (100mW), HR DPSS 561nm (100mW), HR Diode 642nm (150mW)
Cameras:
- sCMOS for SIM,
- EMCCD for PALM
Leica TCS SP2 Confocal and Multiphoton Microscope
Leica TCS SP2 combines an inverted laser scanning confocal microscope with MaiTai multiphoton microscope. It has four confocal laser lines and a multiphoton infrared laser line , an environment chamber and a Eppendorf micromanipulator which make it suitable for high resolution imaging acquisition for cell and tissue samples including live imaging and FRAP.
Objectives:
- HC PL FLUOTAR 10x/0.3,
- HC PL FLUOTAR 20x/0.5,
- HCX PL APO 40x/1.25-0.754 Oil,
- HCX PL APO 63x/1.40-0.60 Oil
Lasers:
- LSM TCS SP2: Coherent Enterprise Ion laser 351nm (2W), Ar 488nm (125mW), HeNe 543nm (1.5 mW),
- HeNe 633 nm (1.5mW)
- MaiTai Multiphoton: Maitai BB-067 Ti:sapphire 650-1100nm (4W)
Camera:
- LEICA DFC420C CCD camera for epifluorescence and brightfield imaging (optional)
Leica DMI4000B Epifluorescence Microscope
Leica DMI4000B is an inverted epifluorescence widefield (WF) microscope suitable for bright field (BF), phase contrast (PH) acquisition and epifluroscence imaging with DAPI, FITC, TRITC, and Texas Red filters on fixed cell and tissue samples (<20µm thickness).
Objectives:
- N PLAN 2.5x/0.07
- HCX PL FLUOTAR 10x/0.3 PH1,
- HCX PL FLUOTAR 20x/0.5 PH2,
- HI Plan I 40x/0.5 PH2,
- HCX PL FLUOTAR 63x/1.25 Oil PH3
Camera:
- LEICA DFC300 FX CCD camera for epifluorescence and BF/PH imaging
Leica DMi8 Epifluorescence Microscope
Leica DMi8 is a semiautomatic inverted epifluorescence widefield (WF) microscope suitable for bright field (BF), phase contrast (PH) acquisition and epifluroscence 2D and 3D imaging with DAPI, GFP, Cy3, and Cy5 filters on fixed cell and tissue samples (<200 µm thickness).
Objectives:
- HC PL FLUOTAR 10x/0.32 PH1,
- HC PL FL L 20x/0.40 CORR, PH1,
- HC PL FL L 40x/0.60 CORR PH2,
- HC PL FLUOTAR 63X/1.30 Oil PH3
Camera:
- LEICA DFC9000 GT sCMOS camera for epifluorescence and BF/PH imaging
Lumascope 720 Microscope
Lumascope 720 is an automatic incubator based, inverted 3-colour (blue, green, red) epifluorescence microscope, with motorized XY stage and auto Z-focus. It is suitable for multiple positions, time lapse live imaging with both phase contrast and fluorescence 2D and 3D mode, up to 10 fps, or 30 fps with reduced frame size.
Objectives:
- Motic EF-N Plan 4x/0.10 (not for imaging),
- Olympus CAch N 10x/0.25,
- Olympus LCAch N 20x/0.40,
- Olympus LCAch N 40x/0.55
Camera:
- CMOS camera for epifluorescence and PH imaging
Chiaro Nanoindenter
The Chiaro nanoindenter is the ideal nanoindentation instrument to explore the micro-mechanical properties of cells, spheroids or other small samples. Therefore, this instrument is purposely built to measure forces on cells and micro-materials while placed on an inverted microscope Leica DMi8. The Chiaro nanoindenter is capable of measuring many mechanical parameters. These include the Young’s Modulus (E), storage (E’) and loss moduli (E”). Moreover, both, static and dynamic indentation can be performed using this instrument, including creep, stress-relaxation and DMA.
Contact:
Prof Martin Knight
email: m.m.knight@qmul.ac.uk
Tel: +44 (0)20 7882 8868