Combined LAPS and SICM for multimodal live cell imaging

A novel instrument will be developed that will revolutionise the ability to monitor cellular processes and cell communication in polarised cells by simultaneously imaging cells apically and basally. This will provide information about apical cell morphology and basal ion concentrations and electrical signals such as cell surface charge and impedance.

ZINCLAPS2

In this project, we propose to develop a novel LAPS setup with high spatiotemporal resolution combined with two-photon fluorescence microscopy that allows imaging of physiological processes with submicron resolution and in real time.

Towards in-vivo electrochemical imaging with flexible, light-addressable electrodes

High-resolution mapping of chemical activity of cells on surfaces is important for the understanding biological processes. We are aiming to develop the first flexible and biocompatible electrochemical imaging chip for in-vivo imaging of cell activity. The groundwork for this will be laid by this PhD project by developing novel organic semiconductor coatings suitable for high-resolution photocurrent imaging and measurement of cell-signalling processes such as cell impedance, cell surface charges, release of metabolites and neurotransmitters.

Materials development for carbon dioxide gas sensors

In this project, a novel carbon dioxide sensor material is developed for use in resistive gas sensors. The research is carried out in collaboration with McGowan Sensor Labs Limited.

Overseas Conference Travel Grant - Analytical Chemistry Trust Fund

Super-Resolution Light-Addressable Potentiometric Sensors (LAPS)

  Super-resolution electrochemical imaging will be developed in this project for the investigation of cell signalling and cell metabolic processes in the cell-surface attachment area of living cells.

Investigation of the interaction of mesenchymal stem cells with aptamer modified surfaces using Light-Addressable Potentiometric Sensors - APTALAPS

This project is to develop a new method to immobilize aptamers onto silicon-on-sapphire (SOS) substrates, to capture stem cells by affinity interaction, and investigate electrochemical images with subcellular resolution using Scanning Photo-induced Impedance Microscopy (SPIM) and Light-Addressable Potentiometric Sensors (LAPS).

A electrochemical and fluorescence microscope for imaging of cell-surface interactions - FLUOLAPS

The project aims to measure cell impedance changes and extracellular potentials of neurons using high-resolution Light-Addressable Potentiometric Sensors (LAPS) and Scanning Photo-Induced Impedance Microscopy (SPIM).

Proof of Concept 2013/14

Scholarship: Norlaily Binti Ahmad

Scanning Photo-induced Impedance Microscopy (SPIM)

AC impedance spectroscopy is a powerful technique for the investigation of electrochemical interfaces and thin and thick films. It provides information about electrochemical reactions, coverage, conductivity and pore formation. However, a major drawback is that the information obtained always represents the properties averaged...

Low cost periodontal disease diagnostic

Periodontal diseases are inflammatory conditions that affect the supporting tissues of teeth and can lead to destruction of the bone support and ultimately tooth loss if untreated. Progression of periodontitis is usually site specific but not uniform, and currently there are no accurate clinical methods for distinguishing sites...

Biosensors based on the degradation of thin polymer films

Disposable biosensors for the detection of enzyme activities based on the enzymatic degradation of thin polymer films have been developed. Film degradation in the presence of an enzyme is monitored using impedance or Quartz Crystal Microbalance.

Biosensor arrays interrogated using SPIM

Disposable sensors based on the degradation of thin films as a result of an enzymatic reaction have been developed into efficient enzyme detectors. Film degradation has traditionally been monitored using Surface Plasmon Resonance (SPR), Quartz Crystal Microbalance (QCM) or classical ac impedance measurements. The enzyme...